nick macpherson answered question 8/23/2007 How Old Are Baby Hamsters When They Open Thier Eyes? When Do I Need To Seperate The Sexes? And One Last Question, Will The "Father" Hampster Attack The Other Male Hampsters If I Put Them All In The Same Cage? They will open their eyes when their about a week old. When the babies are born the father should be remove immediatly because they can hert the babies. You should semporate them in about two to three weeks the sexes.