answered question 4/22/2009 I Took A Pregnancy Test In The Middle Of The Day And It Can Back Negative, And I Do Have All Signs Of Pregnancy, Is It Better To Do A Home Pregnancy Test In The Morning Rather Than In The Middle Of The Day? The best time to take a pregnancy test is the "first pee". Because your pee is the strongest when you wake up on the morning, it will give you the best results.